Question: What is the 1st step of DNA replication?

Answer: The double helix must “unwind”

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Question: What must be broken that is between the nitrogenous bases?

Answer: hydrogen bases

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Question: DNA helicase-

Answer: enzyme that seperates or “unzips” DNA

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Question: place where DNA splits?

Answer: replication fork

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Question: What is the 2nd step of DNA replication?

Answer: nucleotides must be added in the correct order to create a complentary strand

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Question: primase-

Answer: enzyme that adds RNA primers so nucleotides can be attached

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Question: DNA polymerase-

Answer: enzyme that adds nucleotides (and proof reads, and replaces RNA primers with DNA nucleotides)

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Question: DNA polymerase can only read DNA in the ________ direction

Answer: 3’ —> 5’

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Question: it synthesizes a new complementary strand ____

Answer: ‘5 —> 3’

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Question: What is the 3rd step of DNA replication?

Answer: new strand must be sealed up

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Question: DNA ligase-

Answer: enzyme that seals the phosphate-sugar backbone of DNA together

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Question: what was the problem that okazaki solved?

Answer: DNA polymerase can only read DNA in the 3’ —> 5’ direction, it synthesizes a new complementary strand ‘5 —> 3’

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Question: The _____________ proceeds without difficulty because DNA polymerase can read it

Answer: leading strand

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Question: The _____________ must proceed in chunks, called okazaki fragments

Answer: lagging strand

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Question: prokaryote DNA replication-

Answer: DNA replication starts in 1 place continues in 2 different directions until the chromosome is copied

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Question: What is a chromosome also called?

Answer: plasmid

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Question: Eukaryote DNA replication-

Answer: DNA replication occurs at many places at the same time

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