Under What Conditions Would You Adjust The Diaphragm
Question: What year was the first compound microscope invented?
Answer: 1590
Question: Robert Hooke
Answer: In 1655, he used a compound microscope to observe pores in cork, He called them "cells"
Question: Antoine van Leeuwenhoek
Answer: 1st to see single-celled organisms in pond water
Question: magnification
Answer: increase of an object's apparent size.
Question: resolution
Answer: power to show details clearly
Question: 2 things needed to see a clear image with a microscope?
Answer: magnification and resolution
Question: Transmission Electron Microscope(TEM)
Answer: microscope that can magnify up to 250,000x
Question: Compound Light Microscope
Answer: 1st type of microscope, most widely used. Light passes through 2 lenses(eye & objectives). Can magnify up to 2,000x
Question: Electron Microscope
Answer: used to observe VERY small objects: viruses, DNA, parts of cells. Uses beams of electrons rather than light. Much more powerful.
Question: Scanning Electron Microscope(SEM)
Answer: Can magnify up to 100,000x
Question: 4 types of microscopes?
Answer: Compound Light, Electron, Scanning Electron, Transmission Electron
Question: Why are microscopes used?
Answer: Allows you to see small(microscopic) things
Question: arm
Answer: large curved support for the tube; attached at base
Question: base
Answer: weighted bottom of microscope(to prevent tipping over)
Question: clips
Answer: attached to stage to hold slide in place
Question: course adjustment
Answer: large wheel that moves stage up & down; used to focus; used with lower objective
Question: diaphragm
Answer: disc under stage that adjusts amount of light coming in
Question: eye piece
Answer: contains lens at the top end of tube
Question: fine adjustment
Answer: moves stage up and down; this is the best possible focus after course adjustment
Question: magnifying power
Answer: number of times larger the object appears to be under a microscope
Question: nose piece
Answer: bottom end of tube. 3 objectives attached to it. spins to use objectives
Question: objective
Answer: contains lens. may be more than one on a microscope. lowest is short and wide; highest is long and narrow.
Question: stage
Answer: flat area with hole in center. slide is put on it.
Question: tube
Answer: the barrel at which the eyepiece and objective are attached.
Question: An important thing to remember as you turn the high-power objective into place?
Answer: It is retractable. If you hit the slide, the end of the lens will push in so you don't hit the slide.
Question: What should you always remember when you use the coarse adjustment?
Answer: Used to focus microscope. Only used with the low power objective.
Question: Under what conditions would you adjust the diaphragm?
Answer: Used to vary the intensity and size of cone of light that hits the slide.
Question: What should you always remember when handling microscope slides?
Answer: Handle slides at the ends. Never view without a coverslip.
Question: What is the purpose of the stage clips?
Answer: They hold the slides in place
Question: In terms of your eyes, what should you try to learn as you use the microscope?
Answer: Keep both eyes open. Don't let your glasses hit eyepiece lens.
Question: What are the two parts used to carry the microscope?
Answer: The base and the arm
Question: What is the purpose of the coverslip?
Answer: It protects the objective lens from the liquid on the slide.
Question: What is the objective lens used to locate the specimen and first focus?
Answer: Course adjustment is the first to be used to locate the specimen, and only with the low power objective.
Question: What are the chemicals called that are sometimes used to make the specimens visible?
Answer: Called a stain. Methylene Blue is one type of stain.
Question: What should you do if the high power objectives lens touches or breaks the coverslip?
Answer: Tell your teacher.