Question: The goal of aseptic technique is to

Answer: 1. prevent contamination of cultures in the microbiology lab

2. prevent the establishment of an infectious agent in the host.

Question: When incubating a plate it does not matter which way the plate is placed into the incubator as long as it is labeled correctly.

Answer: false

Question: At four hours, you can already easily observe the growth of inoculated bacteria.

Answer: false

Question: Which of the following would indicate that contamination had occurred in a transfer from an

slant to a sterile slant?

Answer: There are red and white colonies on the surface of the slant.

Question: Slants are solid because they contain _____________ which makes them solid at temperatures below 46 degrees Celsius.

Answer: agar

Question: Good aseptic techniques include

Answer: sterilizing inoculating loops.

keeping a lid over an agar plate.

Question: Can you be certain that you made a successful transfer if your sterile broth is turbid after incubation?

Answer: No, the broth will be turbid if it is correctly inoculated, but also if it was contaminated during the inoculation procedure.

Question: A glass tube of solid medium with an angled surface is referred to as a (an)

Answer: agar slant.

slant.

Question: The two main transfer tools that are used when inoculating bacteria from one culture medium to another are called an inoculating _______ and ________

Answer: loop and needle

Question: agar ____________ provide the most surface area for observing the characteristics of bacterial colonies.

Answer: plates/dishes