Under What Conditions Would You Adjust The Diaphragm

Question: What year was the first compound microscope invented?

Answer: 1590

Question: Robert Hooke

Answer: In 1655, he used a compound microscope to observe pores in cork, He called them "cells"

Question: Antoine van Leeuwenhoek

Answer: 1st to see single-celled organisms in pond water

Question: magnification

Answer: increase of an object's apparent size.

Question: resolution

Answer: power to show details clearly

Question: 2 things needed to see a clear image with a microscope?

Answer: magnification and resolution

Question: Transmission Electron Microscope(TEM)

Answer: microscope that can magnify up to 250,000x

Question: Compound Light Microscope

Answer: 1st type of microscope, most widely used. Light passes through 2 lenses(eye & objectives). Can magnify up to 2,000x

Question: Electron Microscope

Answer: used to observe VERY small objects: viruses, DNA, parts of cells. Uses beams of electrons rather than light. Much more powerful.

Question: Scanning Electron Microscope(SEM)

Answer: Can magnify up to 100,000x

Question: 4 types of microscopes?

Answer: Compound Light, Electron, Scanning Electron, Transmission Electron

Question: Why are microscopes used?

Answer: Allows you to see small(microscopic) things

Question: arm

Answer: large curved support for the tube; attached at base

Question: base

Answer: weighted bottom of microscope(to prevent tipping over)

Question: clips

Answer: attached to stage to hold slide in place

Question: course adjustment

Answer: large wheel that moves stage up & down; used to focus; used with lower objective

Question: diaphragm

Answer: disc under stage that adjusts amount of light coming in

Question: eye piece

Answer: contains lens at the top end of tube

Question: fine adjustment

Answer: moves stage up and down; this is the best possible focus after course adjustment

Question: magnifying power

Answer: number of times larger the object appears to be under a microscope

Question: nose piece

Answer: bottom end of tube. 3 objectives attached to it. spins to use objectives

Question: objective

Answer: contains lens. may be more than one on a microscope. lowest is short and wide; highest is long and narrow.

Question: stage

Answer: flat area with hole in center. slide is put on it.

Question: tube

Answer: the barrel at which the eyepiece and objective are attached.

Question: An important thing to remember as you turn the high-power objective into place?

Answer: It is retractable. If you hit the slide, the end of the lens will push in so you don't hit the slide.

Question: What should you always remember when you use the coarse adjustment?

Answer: Used to focus microscope. Only used with the low power objective.

Question: Under what conditions would you adjust the diaphragm?

Answer: Used to vary the intensity and size of cone of light that hits the slide.

Question: What should you always remember when handling microscope slides?

Answer: Handle slides at the ends. Never view without a coverslip.

Question: What is the purpose of the stage clips?

Answer: They hold the slides in place

Question: In terms of your eyes, what should you try to learn as you use the microscope?

Answer: Keep both eyes open. Don't let your glasses hit eyepiece lens.

Question: What are the two parts used to carry the microscope?

Answer: The base and the arm

Question: What is the purpose of the coverslip?

Answer: It protects the objective lens from the liquid on the slide.

Question: What is the objective lens used to locate the specimen and first focus?

Answer: Course adjustment is the first to be used to locate the specimen, and only with the low power objective.

Question: What are the chemicals called that are sometimes used to make the specimens visible?

Answer: Called a stain. Methylene Blue is one type of stain.

Question: What should you do if the high power objectives lens touches or breaks the coverslip?

Answer: Tell your teacher.